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KMID : 0545120010110010164
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Journal of Microbiology and Biotechnology
2001 Volume.11 No. 1 p.164 ~ p.166
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Prediction of the Secondary Structure of the AgfA Subunit of Salmonella enteritidis Overexpressed as an MBP-Fused Protein
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WON, MISUN
KIM, SOYOUN/LEE, SEUNGHWAN/KIM, CHULJUNG/KIM, HYUNSU/JUN, MOOHYUNG/SONG, KYUNG BIN
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Abstract
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To examine the characteristics of the recombinant thin aggregative fimbriae of Salmonella, the AgfA subunit gene was amplified from Salmonella enteritidis using a PCR. The maltose binding protein (MBP)-AgfA fusion protein was overproduced in E. coli and purified. The secondary structure of AgfA was then elucidated from the difference CD spectra. An estimation of the secondary structure of AgfA using the self-consistent method revealed a mostly β-sheet structure.
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KEYWORD
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